Evaluation of Mechanical Allodynia in an Animal Immobilization Model using the Von Frey Method
Objective: The purpose of this study was to evaluate the mechanical allodynia in animals after immobilization and chiropractic manipulation using the Activator instrument (Activator Methods International, Phoenix, Ariz) through the Von Frey test in an animal model that had its hind limb immobilized as a form to induce mechanical allodynia.
Method: Eighteen adult male Wistar rats were used and divided into 3 groups: control group (C) (n = 6) that was not immobilized; immobilized group (I) (n = 6) that had its right hind limb immobilized; immobilized and adjusted group (IAA) (n = 6) that had its right hind limb immobilized and received chiropractic manipulation after. The mechanical allodynia was induced through the right hind limb immobilization. At the end of the immobilization period, the first Von Frey test was performed, and after that, 6 chiropractic manipulations on the tibial tubercle were made using the Activator instrument. After the manipulation period, Von Frey test was performed again.
Results: It was observed that after the immobilization period, groups I and IAA had an exacerbation of mechanical allodynia when compared with group C (P b .001) and that after the manipulation, group IAA had a reversion of these values (P b .001), whereas group I kept a low pain threshold when compared with group C (P b .001).
Conclusion: This study demonstrates that immobilization during 4weeks was sufficient to promote mechanical allodynia. Considering the chiropractic manipulation using the Activator instrument, it was observed that group IAA had decreased levels of mechanical allodynia, obtaining similar values to group C.
(J Manipulative Physiol Ther 2012;35:18-25)